Abstract
A reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the determination of oleanonic acid (OA) content in Chios gum mastic (CGM). A simple method involving methanol extraction of CGM powder followed by basification and ether extraction was developed to isolate the triterpenic fraction including OA. The triterpenic fraction was chromatographed on a Phenomenex Gemini C18 column (150×4.6mm, 5µm) under a simple gradient elution of a mobile phase containing acetonitrile and water at a flow rate of 1.0mLmin-1. The detection wavelength was set at 210nm. Good linearity was achieved in the range of 100.0-1000.0μgmL-1 with r2>0.9993, and the limit of quantification was 32.22μgmL-1. Accuracy measured at three concentration levels was in the range of 93.72-99.56%, while intra-day and inter-day precisions estimated using both OA standard and CGM samples were no more than 2.83 and 4.57% RSD, respectively. Finally, this method was applied to real CGM samples from various batches, revealing that the OA contents were between 88.13 and 100.83μgmg-1. These results suggest that the current method can be applied as an efficient analytical method for quality control of CGM.
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