A simple and rapid section embedding technique for sequential light and electron microscopic examination of individually stained central neurons

Abstract

A method for section embedding of central nervous tissue, and its application to light and electron microscopic examination of neurons stained by intracellular injection of horseradish peroxidase, is described. After primary fixation in aldehydes, thick (10–80 μm) sections are cut on a Vibratome. They are then treated for the histochemical demonstration of peroxidase, postfixed in osmium tetroxide, dehydrated and infiltrated with Spurr's low viscosity resin. Infiltrated sections are embedded between glass slides and coverslips coated with a transparent layer of teflon and polymerized. The resulting mount allows protracted storage and high resolution light microscopic examination of sections. When desired, the glass components can be removed and the specimen cemented to a blank block for thin sectioning and electron microscopic examination.