A Simple and Rapid Procedure for the Detection of Self-Compatible Individuals in Japanese Apricot (Prunus mume Sieb. et Zucc.) Using the Loop-Mediated Isothermal Amplification (LAMP) Method

Abstract

Japanese apricot (Prunus mume Sieb. et Zucc.) exhibits S-RNase-based gametophytic self-incompatibility as do other Prunus species. Both self-incompatible and self-compatible Japanese apricot cultivars are grown commercially in Japan. These self-compatible cultivars are shown to have a common S-haplotype called Sf that contains Sf-RNase and SFBf (S-haplotype-specific F-box protein). This study describes a simple and rapid detection of SFBf, in Japanese apricot, based on loop-mediated isothermal amplification (LAMP) method. A set of 4 primers, F3, B3, FIP, and BIP primer, were designed from the exon and the putative inserted sequence of SFBf. Optimal reaction time at 63 C was determined to be 90 minutes. It appeared that the LAMP method combined with the ultrasimple DNA extraction efficiently detected SFBf. The advantage of the marker-assisted selection of self-compatibility based on the LAMP method was discussed.