A simple and rapid method for the differentiation of the members of the genus Klebsiella.

Abstract

Nitric oxide (NO) is an endogenous, diffusible, transcellular messenger shown to affect regulatory and signaling pathways with impact on cell survival. Exposure to NO can impart direct post-translational modifications on target proteins such as nitration and/or nitrosylation. As an alternative, after interaction with oxygen, superoxide, glutathione, or certain metals, NO can lead to <i>S</i>-glutathionylation, a post-translational modification potentially critical to signaling pathways. A novel glutathione <i>S</i>-transferase π (GSTπ)-activated pro-drug, <i>O</i>²-{2,4-dinitro-5-[4-(<i>N</i>-methylamino)benzoyloxy]phenyl}1-(<i>N</i>,<i>N</i>-dimethylamino)diazen-1-ium-1,2-diolate (PABA/NO), liberates NO and elicits toxicity in vitro and in vivo. We now show that PABA/NO induces nitrosative stress, resulting in undetectable nitrosylation, limited nitration, and high levels of <i>S</i>-glutathionylation. After a single pharmacologically relevant dose of PABA/NO, <i>S</i>-glutathionylation occurs rapidly (&lt;5 min) and is sustained for ∼7 h, implying a half-life for the deglutathionylation process of approximately 3 h. Two-dimensional SDS-polyacrylamide gel electrophoresis and immunoblotting with a monoclonal antibody to <i>S</i>-glutathionylated residues indicated that numerous proteins were <i>S</i>-glutathionylated. Subsequent matrix-assisted laser desorption ionization/time of flight analysis identified 10 proteins, including β-lactate dehydrogenase, Rho GDP dissociation inhibitor β, ATP synthase, elongation factor 2, protein disulfide isomerase, nucleophosmin-1, chaperonin, actin, protein tyrosine phosphatase 1B (PTP1B), and glucosidase II. In addition, we showed that sustained <i>S</i>-glutathionylation was temporally concurrent with drug-induced activation of the stress kinases, known to be linked with cell death pathways. This is consistent with the fact that PABA/NO induces <i>S</i>-glutathionylation and inactivation of PTP1B, one phosphatase that can participate in deactivation of kinases. These effects were consistent with the presence of intracellular PABA/NO or metabolites, because cells overexpressing MRP1 were less sensitive to the drug and had reduced levels of <i>S</i>-glutathionylated proteins.