Abstract
Per- and polyfluorinated alkyl substances (PFASs) are ubiquitous environmental contaminants, present in the environment and in the human body. They have raised global concern because of their diffusion in the environment, particularly in water, causing cases of human overexposure due to consumption of contaminated drinking water. Human biomonitoring is the most effective way to characterize human exposure to PFASs, and it is important that as many labs as possible could easily perform this kind of analysis. Analytical methods for quantitation of PFAS mixtures in human serum have been developed, but most of them required materials that are not always easily available in all the laboratories. This paper describes a very simple and accessible HPLC MS/MS method of analysis and quantification of 13 perfluorocarboxylic acids and perfluorosulfonate compounds (belonging to the class of per- and polyfluorinated alkyl substances (PFASs)) in human serum. Method development data provide detailed descriptions of the optimization process in terms of sample preparation, laboratory analysis of human serum samples, determination of analytes by HPLC MS/MS, and describing the pump gradient time, working conditions, and acquisition.
Highlights
Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are a class of synthetic organic chemicals containing fully (per) or partly (poly) fluorinated carbon chains with different functional groups used since 1950s in various industrial and commercial products (fire foams, clothes, protective coatings for carpets, pots, paints, and insecticides) [1]
Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are a class of synthetic organic chemicals containing fully or partly fluorinated carbon chains with different functional groups used since 1950s in various industrial and commercial products [1]. They have spread in the environment and have entered the food chain [2,3,4,5,6]. eir chemical-physical properties are thermal and chemical resistance, impermeability to oil and water, and surfactant properties. ese chemical-physical properties combined with high industrial production and frequent and widespread use have made PFAS persistent contaminants capable of spreading in the environment without degrading but bioaccumulating and bioconcentrating in the food chain [7, 8]
Sampling is usually performed by Italian local sanitary units in charge for blood withdrawal, which we provide with specific instructions to avoid PFAS contamination, contaminations deriving from the presence of teflon-coated materials or similar
Summary
Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are a class of synthetic organic chemicals containing fully (per) or partly (poly) fluorinated carbon chains with different functional groups used since 1950s in various industrial and commercial products (fire foams, clothes, protective coatings for carpets, pots, paints, and insecticides) [1]. We ordinarily use the method hereafter described for the analysis of serum samples within the framework of various national and international projects and in interlaboratory comparison exercises [10, 14,15,16,17,18], Ring Exercise Test for Persistent Organic Pollutants in Human Serum https:// www.inspq.qc.ca/en/ctq/eqas/amap/description [19].
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