Year-end Sale % OFF 
Abstract
In spite of the possibility of analysing large samples and the rapidity of Polymerase Chain Reaction (PCR) technology, the usual protocols for DNA plant extraction remain time-consuming, slow and involves use of hazardous chemicals. To lessen labor, time or cost of DNA extraction, a simple and instant method for genomic DNA extraction from leaf tissue of safflower (Carthamus tinctorius L.) leaf is established. Small quantity of tissue material (typically3-5mg) was ground in a centrifuge tube using plastic pestle in extraction solution. ExtractedDNA was suitable for PCR analysis, without centrifugation. The feasibility of this method was confirmed by testing molecular markers and transgene detection. This method requires less than 1 mg of plant tissue stored frozen or used fresh and is useful for molecular marker analyses as well as transgene detection.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
