Abstract
First-derivative synchronous spectrofluorimetry was applied to the simultaneous resolution of propranolol and hydralazine in pharmaceutical preparations. Chemical and instrumental parameters were optimized for the determination of mixtures of these drugs. The analysis is performed by measuring the first-derivative synchronous spectra at certain selected pairs of excitation and emission wavelengths where the responses exhibit no contribution to each other. The optimization of the synchronous scanning path was accomplished by analysis of the three-dimensional excitation-emission matrices.
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