Abstract
A simple, relatively inexpensive, and effective method for the control of relative humidity in an environmental chamber was developed and evaluated. Relative humidity was maintained at 90% ± 0.4% and 95.2% ± 0.5% through the use of a micrologger-controlled ultrasonic mister that cycled between relatively narrow set points. To maintain consistent relative humidity, misters were activated at approximately 5- and 3-min intervals at 90% and 95% relative humidity, respectively. Air temperatures of 20–28°C were maintained at ±0.5°C by regulation of growth room controls. This environmental chamber has considerable utility for investigations of the influence of relative humidity and water potential on plants and plant-associated microorganisms.
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