Abstract
A new procedure is described for the sequence determination of oligonucleotides produced by digestion of RNA with pancreatic RNase A. The oligonucleotide is treated with spleen exonuclease and all intermediates are resolved by thin-layer chromatography on polyethyleneimine plates. On the basis of the increase in mobility it can be decided for each successive step whether a Gp- or an Ap-residue has been removed by reference to a calibration grid. The method is very simple and can easily be applied to a large number of samples. An amount of 32P-radioactivity corresponding to 40 dpm/nucleotide is sufficient for analysis.
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