A simple and fast method for the simultaneous determination of Imperatorin, Isoimperatorin and Umbelliferone at β-CD–GO composite film modified GCE

Abstract

β-CD–GO composite film was immobilized on a glassy carbon electrode (GCE) to develop an electrochemical sensor for simultaneous determination of the isomers of Imperatorin (IMP) and Isoimperatorin (IIMP) and Umbelliferone (UB) for the first time. The electrochemical behaviors of the three components were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The peak-to-peak potential differences between IMP and IIMP, IMP and UB, and IIMP and UB were 0.135, 0.540 and 0.405 V, respectively. The calibration curves for IMP, IIMP and UB were obtained in the range of 2.5–90.0 μM, 3.0–90.0 μM and 0.8–20.0 μM with the limits of detection of 0.5, 0.8 and 0.05 μM (S/N = 3), respectively. The modified electrode was successfully applied for the simultaneous determination of IMP, IIMP and UB in traditional Chinese medicine Angelica dahuricae Radix and Duliang Diwan with excellent recoveries as confirmed by HPLC method.