Abstract
A simple and fast manual centrifuge was developed to spin down solutions in 96-well polymerase chain reaction (PCR) plates. A commercially available salad spinner was utilized for this purpose. Acceleration and deceleration of the centrifuge were faster than those of a conventional electric centrifuge using 96-well PCR plates. Solutions in a 96-well PCR plate settled quickly after centrifuging for only 3 s. This lightweight centrifuge can be stored under a laboratory bench or on a shelf and can be put on the bench only when required, whereas the electric centrifuge is immobile due to its weight and the requirement of electric cables. This simple centrifuge is inexpensive, requires minimal effort for making, and can be used anywhere.
Highlights
Genotyping by polymerase chain reaction (PCR) is an important technique for screening mutants in various organisms [1,2,3] and genome editing by CRISPR/Cas9 [4,5,6,7]
PCR solutions in 96-well PCR plates were sufficiently centrifuged using the manual centrifuge for 3 s (Figure 3A)
DNA fragments from these centrifuged PCR mixtures could be clearly visualized by DNA agarose gel electrophoresis (Figure 4) [19]
Summary
Genotyping by polymerase chain reaction (PCR) is an important technique for screening mutants in various organisms [1,2,3] and genome editing by CRISPR/Cas9 [4,5,6,7]. Escherichia coli colony-PCR is an efficient technique for screening positive colonies [8,9,10], especially for high-throughput-cloned E. coli plasmids expressing recombinant proteins [11]. Several commercially available electric centrifuges can be used for this purpose. These centrifuges require a few seconds for acceleration and deceleration to spin the PCR solutions down in 96-well PCR plates. A high-speed hand-powered paper centrifuge was developed for separation of plasma from whole blood [18]. I developed a manual centrifuge to spin solutions down in 96-well PCR plates for conventional PCR screening, thereby significantly improving the commercially available salad spinner. The manual centrifuge is lightweight, portable, and usable in any space
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