A Simple and Facile Glucose Biosensor Based on Prussian Blue Modified Graphite String

Seung Ho Lee,Gi-Ja Lee,Hun-Kuk Park,Joo-Ho Chung

doi:10.1155/2016/1859292

Abstract

This work describes the string sensor for the simple and sensitive detection of glucose which is based on Prussian blue (PB) modified graphite utilizing dipping. First, PB modified graphite (PB-G) strings are characterized by physical and electrochemical techniques to optimize the PB-G layer thickness. Then, glucose oxidase (GOx) is immobilized on PB-G string electrode with biocompatible chitosan overlayer (Chi/GOx/PB-G). The Chi/GOx/PB-G string electrode exhibits a sensitivity of 641.3 μA·mM−1·cm−2to glucose with a linear range of 0.03 to 1.0 mM (R2=0.9957) and a rapid response time (<3 s). Moreover, the Chi/GOx/PB-G string electrodes are less sensitive to common interference materials such as ascorbic acid, uric acid, galactose, and acetaminophen than to glucose. The Chi/GOx/PB-G string electrodes also show excellent reproducibility (<5% RSD). Therefore, our Chi/GOx/PB-G string electrodes can be simple, robust, and reliable tools for glucose sensing which can avoid complicated and difficult multistep fabrication processes. In addition, we expect that they have many potential applications in fields ranging from health care to food analysis, in particular where single use is favorable.

Highlights

Current demands for point-of-care testing devices for clinical diagnostics and on-site environmental monitoring have attracted great attention in terms of developing flexible, lightweight, low-cost, and wearable sensors [1, 2]
We investigate the effect of the dipping number on the electrochemical property of Prussian blue (PB)-G string electrode
The PB modified graphite (PB-G) coating on string was more densely packed after the fifth dipping, and the resistance as well as the amounts of PB was sufficient to act as an electrode for electrochemical sensing

Summary

Introduction

Current demands for point-of-care testing devices for clinical diagnostics and on-site environmental monitoring have attracted great attention in terms of developing flexible, lightweight, low-cost, and wearable sensors [1, 2]. Smart fabrics with biomonitoring functionality have a lot of potential in health care, sports, and defence applications. Inkjet or screen printing is an attractive deposition technology for the fabrication of PB modified sensor, it still requires the film substrates and special instruments such as inkjet or screen printer. Dipping technique is the most convenient way of the processing of fabric, yarn, or string because it is simple and of low cost and has rapid fabrication process without needing special instrument

Methods

Results

Conclusion