Abstract

A simple squash technique has been proposed to study the somatic chromosomes of crucifers. Essentially the technique consists of pretreatment with alpha-bromonaphthelene followed by fixation in methanol, acetic acid (1:1) and Newcomer's fluid, and staining with aceto-orcein. Consistent and excellent preparations have been obtained in rapeseed, cabbage, cauliflower, radish, turnip and Iberis spp.

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