Abstract
Elution of gonadotropins and other glycoproteins from concanavalin A has been previously performed with competitive ligands such as α-methyl- d-mannopyranoside and α-methyl- d-glucopyranoside. In comparative studies we found that 1 m NH 4OH was a highly effective eluent solution and gave nearly complete recovery of gonadotropin as revealed by protein contents, immunological, and biological activities. Advantages of 1 m NH 4OH over the sugar derivatives are its low cost and volatile nature, and its ability to irreversibly inactivate concanavalin A. The use of this system permits further steps of purification to be performed directly or after lyophilization without dialysis.
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