A signal transduction system in Streptomyces coelicolor that activates expression of a putative cell wall glycan operon in response to vancomycin and other cell wall‐specific antibiotics

Abstract

This article was published in Mol Microbiol (2002) 44(5), 1199–1211. The authors wish to make the following correction. The panel labelled “ΔsigE” in Fig. 3 (B) was in error, and is in fact the data from a ΔcseA mutant, correctly published as such in Hutchings et al. (2006), and the ΔsigE panel has therefore never been published. To clarify, the complete data set, originally split between the two papers, is reproduced here. Figure. Induction of the sigE promoter (sigEp) in its native chromosomal context by vancomycin. S1 nuclease protection analysis of the sigE transcript in S. coelicolor M600 (wt), J2130 (ΔsigE), J2172 (ΔcseA), and J2142 (ΔcseB::hyg) grown to mid-late exponential phase in NMMP liquid medium containing 2 mM MgCl2 and exposed to 10 µg/ml vancomycin. RNA was extracted from samples taken immediately prior to the addition of vancomycin and at subsequent 30 min intervals. Relative signal strengths were quantified on a Phosphorimager; each signal is expressed as a percentage of the strongest signal.