Abstract
A green electrodeposition method was firstly employed for the synthesis of round hairbrush-like gold nanostructure in the presence of cadaverine as a size and shape directing additive. The nanostructure which comprised of arrays of nanospindles was then applied as a transducer to fabricate a signal-on built in-marker electrochemical aptasensor for the detection of human prostate-specific antigen (PSA). The aptasensor detected PSA with a linear concentration range of 0.125 to 128 ng mL−1 and a limit of detection of 50 pg mL−1. The aptasensor was then successfully applied to detect PSA in the blood serum samples of healthy and patient persons.
Highlights
Prostate cancer (PCa) is the most common cancer in men and is the second-leading cause of cancer mortality in men
Prostate-specific antigen (PSA) is a biomarker that is most widely employed for the detection of PCa2
It has been shown that PSA is the most validated biomarker for the early detection of prostate cancer and monitoring the disease recurrence after treatment[3]
Summary
All chemicals were of analytical grade from Scharlau (Spain) or Merck (Germany). All solutions were prepared by redistilled water. A specific aptamer sequence for PSA which was modified with methylene blue (MB) was employed with following sequence. It has been reported that this sequence has a high affinity to PSA46 and modified with MB in this study: 5′ SH-(CH2)[6] TT TT TA AT TA AA GC TC GC CA TC AA AT AG CT TT-3′-MB. The aptamer was purchased from Bioneer (Korea). PSA, hemoglobin and bovine serum albumin were purchased form Sigma (USA). The aptamer stock solutions were prepared with a 20 mmol dm−3 Tris-HCl buffer, pH 7.4 solution (Tris) and kept frozen
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