Abstract
Global biodiversity, especially fish, has experienced a decline, this occurs as a result of over-exploitation, the presence of introduced fish species and climate change. This condition makes researchers look for solutions to overcome these problems by using cryopreservation techniques. The main purpose of cryopreservation is to store, maintain, and ensure the survival of genetic material, so that using cryopreservation techniques can maintain the viability and function of gamete cells both immunologically, biologically and physiologically. The success of the cryopreservation technique has made this technique widely developed in various species of living organism including fish. This article summarizes and reviews the history of the development of cryopreservation of animal species with specific focus on fish.Keywords:CryopreservationHistoryDepikEndemic species
Highlights
Biodiversity is one of components supporting survival of human life, including for food, development of medicines, science, and even energy
Cryopreservation is a process when germplasm such as cells and tissues are preserved in low temperatures (-196oC) (Agarwal, 2011)
Some of antioxidants have been tested in fish sperm cryopreservation such as glutathione in P. tawarensis (Muthmainnah et al, 2019), superoxide dismutase in brown trout Salmo trutta (Lahnsteiner et al, 2011), Uric acid, ascorbic acid, α- tocopherol, methionine in Rainbow trout Oncorhynchus mykiss (Kutluyer et al, 2014)
Summary
Biodiversity is one of components supporting survival of human life, including for food, development of medicines, science, and even energy. While cryopreservation of human sperm was first reported by Sherman and Bunge (1953), until 1963, they continued to develop methods of preserving human spermatozoa by following various cryopreservation protocols and freeze-drying techniques. They found the standard method for human sperm preservation stored in liquid nitrogen at -196°C. Until 1963, Sherman and Bunge continued to develop methods of preserving human sperm by seeking various cryopreservation protocols and freeze-drying techniques, resulting in storage technique for human sperm preservation is in liquid nitrogen with a temperature of -196oC
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