Abstract
Skin wound, a common form of skin damage in daily life, remains a serious challenge in clinical treatment. Bioactive peptides with high efficiency have been considered as potential therapeutic candidates for wound healing. In this report, a novel short linear peptide, with mature peptide sequence of ‘GLLSGINAEWPC’ and no obvious similarity with other known bioactive peptides, was identified by genomic method from the skin of odorous frog, Odorrana andersonii. Our results suggested that OA-GL12 (OA: abbreviation of species (O. andersonii), GL: two initial amino acids, 12: peptide length) obviously accelerated the scratch-healing of keratinocytes and human fibroblasts in a time- and concentration-dependent manner. Meanwhile, OA-GL12 showed significant effect in promoting the wound healing on the full-thickness skin wound model. Inflammatory assay results demonstrated that OA-GL12 induced the secretion of tumor necrosis factor (TNF) and transforming growth factor β1 (TGF-β1) on murine macrophage cell line (RAW264.7), which might explain the powerful accelerating capacity of wound healing. Moreover, results also indicated that epidermal growth factor receptor (EGFR) was involved in the mechanisms underlying the scratch-healing promoting activity of OA-GL12. In addition, OA-GL12 showed obvious free radical scavenging activity. Results supported that OA-GL12 did not exert risk in acute toxicity, hemolytic activity, and direct antibacterial activity. The remarkable effect of OA-GL12 on promoting wound healing verified in this research made it potential to be a novel template for the development of wound healing-promoting agents.
Highlights
Human skin wounds remain a major and snowballing threat to public health and the economy [1]
Our results suggested that OA-GL12 could be a novel template for the development of wound healing-promoting drug
This mature peptide is a linear motif, which is quite different from the cyclic motif of Nigrocin-2S, it showed no obvious sequence similarity with other bioactive frog peptides and was considered to be novel and named as OA-GL12 according to our previous reports (OA: abbreviation of species, GL: two initial amino acids, 12: peptide length) [13,14]
Summary
Human skin wounds remain a major and snowballing threat to public health and the economy [1]. Under some conditions (diabetes, infection, et al.), the process of wound healing will be delayed and mostly inflicted chronic wounds [5,6]. Accelerating the wound healing is vital to the body. Traditional wound healing drugs, including growth factors, cytokines, chemical compounds derived or developed from plants, and other immunomodulatory factors, were difficult to translate these therapies for chronic wound healing into the clinic [7]. Compared with those drugs with high cost, low activity, safety and delivery problems, bioactive peptides with high activity, specificity, and stability have aroused considerable interest in the related field of research [8–10]
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