Abstract
Active peptide from shark liver (APSL) is a cytokine from Chiloscyllium plagiosum that can stimulate liver regeneration and protects the pancreas. To study the effect of orally administered recombinant APSL (rAPSL) on an animal model of type 2 diabetes mellitus, the APSL gene was cloned, and APSL was expressed in Bombyx mori N cells (BmN cells), silkworm larvae and silkworm pupae using the silkworm baculovirus expression vector system (BEVS). It was demonstrated that rAPSL was able to significantly reduce the blood glucose level in mice with type 2 diabetes induced by streptozotocin. The analysis of paraffin sections of mouse pancreatic tissues revealed that rAPSL could effectively protect mouse islets from streptozotocin-induced lesions. Compared with the powder prepared from normal silkworm pupae, the powder prepared from pupae expressing rAPSL exhibited greater protective effects, and these results suggest that rAPSL has potential uses as an oral drug for the treatment of diabetes mellitus in the future.
Highlights
Diabetes mellitus is a chronic metabolic disease characterized by increasing glucose blood levels
Our study demonstrates for the first time recombinant APSL (rAPSL) synthesis using the silkworm baculovirus expression vector system
RAPSL was expressed by rBv-Active peptide from shark liver (APSL) using the Bac-to-Bac baculovirus expression system. rBv-APSL successfully infected silkworm pupae, and the infected silkworm pupae were used as a bioreactor to express rAPSL
Summary
Diabetes mellitus is a chronic metabolic disease characterized by increasing glucose blood levels. It is caused by the interaction of both genetic and environmental factors [1]. Type 2 diabetes mellitus is characterized by inadequate insulin secretion and insulin resistance (IR) and causes serious harm to humans, often leading to metabolic disorders involving carbohydrates, proteins and fats [1,2]. These disorders can result in serious complications involving blood vessels, nerves, the heart, kidneys and other organs. Zhang et al showed that a protein expressed by a silkworm pupae bioreactor could act as an active cytokine when orally administered [3]
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