Abstract
Abstract. Based on the sequences of the bovine amelogenin genes, we have designed a protocol for sexing DNA samples of wild ruminants. Basically the protocol consists on the co-amplification of two specific fragments, one from Y-chromosome and one for the X chromosome, making the use of a PCR control unnecessary. It has been demonstrated to be useful for sex identification in a total of 164 samples belonging to six different wild ruminant species. We propose adding to the census procedure commonly based in faecal groups counting, the faecal sampling and application of the protocol design here, to estimate the sex ratio.
Highlights
Sex identification of unknown samples is useful for natural population managing, allowing the knowledge of the sex ratio
We have established a simple and accurate method to determine the sex of unknown wild ruminant samples based on a PCR method, using bovine amelogenin primer sequences
This method can be applied to other domestic ruminant species other than bovine, whose sequence was used to design this protocol
Summary
Sex identification of unknown samples is useful for natural population managing, allowing the knowledge of the sex ratio. In mammals females are the homogametic sex and have two copies of the X chromosome. Males are the heterogametic sex and have only one copy of the X chromosome and one copy of the Y chromosome. Different approaches, based on PCR methods, have been used in order to determine the sex. Some of the existing protocols are only based on the PCR-detection of Y chromosome specific sequences, such as genes: SRY (TAKAHASHI et al, 1998; MARA et al, 2004), and TSPY (LEMOS et al, 2005); or repeated sequences (SCHROEDER et al, 1990; BREDBACKA and PEIPPO, 1995; KAGEYAMA et al, 2004). Sexing protocols need a PCR product being out of the Y chromosome, as a positive control of template (DNA) in the sample, or an X chromosome specific fragment
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