Abstract
Luculia gratissima (Wall.) Sweet (Rubiaceae) is a perennial shrub distributed in the southeast margin of the Tibetan Plateau in southwest China and adjacent region of Nepal and Myanmar. The plant is a distylous species with reciprocally placed stigmas and anthers in each floral morph. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing (FIASCO) repeats protocol, 19 primer sets were identified in two wild populations. Of these primers, 10 displayed polymorphisms and nine were monomorphic. The number of alleles per locus ranged from two to five, values for observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.289 to 0.760, with averages of 0.303 and 0.555, respectively. These microsatellite loci will facilitate further studies on breeding system, gene flow patterns, and population structure of L. gratissima and its allied species.
Highlights
Luculia gratissima (Wall.) Sweet (Rubiaceae) is an evergreen perennial shrub distributed in the southeast margin of the Tibetan Plateau in southwest China and adjacent region of Nepal and Myanmar at altitudes between 800 and 2400 m [1,2]
We have developed and characterized 19 microsatellite markers for L. gratissima using the
Nineteen microsatellite loci successfully amplified in L. gratissima for 33 microsatellite loci and 10 of them were polymorphic amplification, the remaining nine microsatellite loci were monomorphic as a result of capillary gel (Table 1)
Summary
Luculia gratissima (Wall.) Sweet (Rubiaceae) is an evergreen perennial shrub distributed in the southeast margin of the Tibetan Plateau in southwest China and adjacent region of Nepal and Myanmar at altitudes between 800 and 2400 m [1,2]. It is very interesting that the species is distylous with complementary positioning of stigmas and anthers in the two floral forms [3]. Previous studies suggest that the floral syndrome of distylous species would facilitate disassortative mating during pollinator visitation [4,5]. The potential functions of distyly are not well understood, and DNA microsatellites would provide a precise molecular marker to trace the characteristic of pollination patterns by parentage analysis. We have developed and characterized 19 microsatellite markers for L. gratissima using the. Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of sequences containing repeats (FIASCO) [6], which will be used for further studies of breeding system, gene flow patterns, and population structure
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