Abstract
A multiplex PCR assay was developed for the identification of polymorphic microsatellite loci (18) for the Algerian mouse, Mus spretus, using 454 GS-FLX Titanium pyrosequencing. The utility of these markers was evaluated in 184 individuals from for different Moroccan populations. Number of alleles per locus ranged from 10 to 39, with expected and observed heterozygosities ranging from 0.256 to 0.947 and from 0.259 to 0.926 respectively. None departures from Hardy–Weinberg equilibrium were observed at all loci. These markers will be valuable tools for population genetic studies in conservation management.
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