Abstract
Non-destructive and interference-free monitoring of pesticide residue on the surface of fruits is still a challenge. Herein, a SERS aptasensor based on a flexible substrate was established for effective carbendazim (CBZ) detection on apple peel. In this sensor, electrospun PVDF/CQDs film served as a flexible supporting substrate. AuNS@Ag was liquid–liquid self-assembled on the PVDF/CQDs film to form a uniform and highly active SERS substrate. During the detection process, aptamers specifically capture the CBZ molecules, while nitrile-mediated Raman tag (MMBN) linked to AuNPs provided optical anti-interference signals. The results showed that the developed sensor had high sensitivity, selectivity, reproducibility, and stability for CBZ detection. Importantly, the flexibility of the SERS substrate helped the sensor realize non-invasive CBZ detection at a concentration as low as 1.20 ng/cm2 on apple peel, which is much lower than the maximum residue limits of CBZ in apples.
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