Abstract
In pH 6.6 Na2HPO4-NaH2PO4 buffer solution and in the presence of NaCl, nanogolds were aggregated to form stable aggregated-nanogolds (ANG). The molecular probe of coomassie brilliant blue G-250 (CBBG) adsorbed on the surface of ANG to form CBBG -ANG conjugates that produce the strongest surface-enhanced resonance Raman scattering (SERRS) peak at 1171 cm-1. Under the optimized conditions, the increased intensity ΔI was linear to CBBG concentration in the range of 0.29×10-7 - 4.68×10-7 mol/L, with the regression equation of ΔI = 0.86C - 17.1. A fast and sensitive method for trace CBBG was developed.
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