Abstract
An optical multichannel absorbance detection system suitable for micro-HPLC is described. The detection system includes a flow cell with detection volume of 30 nL and path length of 12 mm. A fiber optic spectrometer with charge-coupled device array is used to collect spectral information at 1 Hz frequency. Signal-to-noise ratios are enhanced through the use of large bandwidth total absorbance signals, while linearity and spectral resolution are maintained. Theoretical predictions of bandwidth dependent signal-to-noise ratios of the total absorbance signal are compared to experimental observations, showing that optimum total absorbance signal bandwidths occur at 2.8 σabs for a Gaussian absorption band under light noise limited conditions. This bandwidth is much larger than bandwidths used in most commercial detectors. The detection system is applied to the separation of 2,4-dinitophenylhydrazones of organic carbonyls sampled from tropospheric air samples. For these hydrazones, a bandwidth of 181 nm is used...
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