A Sensitive Signal-on Supersandwich DNA Biosensor Based on the Enhancement of Poly(aniline-luminol) Nanowires Electrochemiluminescence by Ferrocene.

Abstract

A signal-on supersandwich type of electrochemiluminescence (ECL) DNA biosensor was developed based on the poly(aniline-luminol) nanowires (PALNWs) modified electrode and enhancement of ferrocene (Fc) on ECL of luminol. Aminated capture DNA was covalently linked to the PALNWs on the electrode surface by the crosslinking of glutaraldehyde. In presence of target DNA, its 3' terminus hybridizes with the capture probe and the 5' terminus hybridizes with ferrocene labeled DNA (Fc-DNA) to form a long DNA concatamer supersandwich structure. The ECL intensity of the prepared biosensor was clearly improved by increasing the concentration of target DNA due to the enhancement of ferrocene on luminol ECL. The difference of the ECL intensity in the absence and presence of target DNA was used to monitor the hybridization event. The difference of ECL linearly increased with the logarithm of target DNA concentration in the range from 1.0 × 10-16 - 1.0 × 10-8 mol L-1 with a detection limit of 5.8 × 10-17 mol L-1. The sensor had high sensitivity and wide linear relationship for the detection of target DNA.