Abstract
A rapid, sensitive and accurate liquid chroma-tographic tandem mass spectrometric method is described for the determination of Mimosine in Mimosa pudica Linn. whole plant powder. Mi-mosine was extracted from the plant using 1.0% HCl in water. The chromatographic separation was achieved using a Thermo Hypurity C18 (50 x 4.6 mm) 5.0 μ column interfaced with a triple quadrapole mass spectrometer. The mobile phase consisted of a mixture of Methanol: 10 mM ammonium formate buffer whose pH was ad-justed to 3.00 ± 0.05 with formic acid (80:20, v/v) and was delivered at a flow rate of 1.0 mL min-1. Electrospray ionization (ESI) source operated in the negative ion mode was used for the quantitation. Detection was performed using an Applied Biosystems Sciex API 3200 Mass spec-trometer. The method was found to be simple, precise, accurate, fast, specific and sensitive and can be used for routine quality control analysis of Mimosine in Mimosa pudica Linn.
Highlights
Mimosa pudica Linn. (Fam. -Leguminosae) is commonly known as Sensitive plant in English and Lajvanti or Chuimui in Hindi language
Phytochemical screening has revealed that the plant contains Mimosine, stigmasterol, leucoanthocyanidin, D-xylose and D-glucuronic acid, norepinephrine, D-pinitol, linoleic acid, oleic acid, palmitic acid, stearic acid, -sitosterol and crocetin dimethyl ester
Mimosine is used for treating the cutaneous effects of psoriasis and related skin disorders [6]
Summary
Mimosa pudica Linn. (Fam. -Leguminosae) is commonly known as Sensitive plant in English and Lajvanti or Chuimui in Hindi language. In Ayurvedic system of medicine, Mimosa pudica Linn. Phytochemical screening has revealed that the plant contains Mimosine (alkaloid), stigmasterol, leucoanthocyanidin, D-xylose and D-glucuronic acid, norepinephrine, D-pinitol, linoleic acid, oleic acid, palmitic acid, stearic acid, -sitosterol and crocetin dimethyl ester. The major compound present in Mimosa pudica Linn. Mimosine is used for treating the cutaneous effects of psoriasis and related skin disorders [6]. It is less soluble in methanol and ethanol, insoluble in other organic solvents, but sparingly soluble in water. It is soluble in dilute acid and base.
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