Abstract
A radioimmunoassay for 6-keto-prostaglandin F 1α (6-keto-PGF 1α) is reported. The mean least detectable mass of 6-keto-PGF 1α is 1.45 pg and the mean mass of added 6-keto-PGF 1α required to displace zeropoint binding by 50% is 94.2 pg. Cross-reactivities of the antiserum with all the prostaglandins and related compounds tested are < 0.1% except for PGE 2 and PGF 2α which are respectively 0.16% and 0.80%. The assay is both accurate and reproducible and measured levels are in good agreement following extraction of 0.25–4.0 ml of plasma. Mean circulating concentrations of 6-keto-PGF 1α five non-pregnant humans, rhesus monkeys and sheep were 115, 65 and 25 pg/ml respectively.
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