Abstract
This paper presents a portable fluorescence detector with a tested limit of detection (LOD) at 0.4 nM of the fluorescein solution. This detector was prototyped with a miniaturized integrating sphere that couples a sample-filled glass flow-cell, a 450 nm laser diode, a 550 nm long-pass optical filter, and a light sensor. The integrating sphere is shown to improve the sensitivity and resolution of detection by collecting all the fluorescence emanation. A comparative study with two integrating spheres of 0.75-in. diameter, reflective versus black, shows that the former enables (1) a larger amount of irradiance at the light sensor, (2) a lower LOD, and (3) better resolution of detection. An estimation of the battery lifetime is given for various scenarios of operating this fluorometer for measurements.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
