A Sensitive Multilayered Structure Suitable for Biosensing on the BioDVD Platform

Abstract

Several technologies are currently available for the analysis of biomolecular interactions with high sensitivity and efficiency. However, these instruments are invariably expensive and, thus, are not suitable for bedside analyses. To circumvent this issue, we have previously reported a BioDVD platform that allowed us to use a DVD mechanism to monitor various biomolecular interactions [Gopinath et al., 2008, ACS Nano 2, 1885-1895]. In the present study, to improve the sensitivity of the BioDVD platform for various analyses, we have performed computer simulations to optimize the ZnS-SiO(2) layer thicknesses and determined an optimized optical interferometric response after adjusting the ZnS-SiO(2) layer thickness to 65 and 60 nm for the inner and outer layer thicknesses, respectively. Biomolecular interaction analyses performed with the optimized BioDVD disks revealed a 3-fold improvement in the sensitivity, compared to our previously reported multilayered structure. In this study, we have also shown that the BioDVD platform is suitable not only for analyzing nucleic acid hybridization and interactions between RNA-small ligands and RNA-proteins, but also for antigen-antibody interactions. Furthermore, our evaluations revealed that each sample required no more than 10 tracks of data to analyze the biomolecular interactions on the BioDVD platform, which permits a greater number of spots per BioDVD disk and also reduces the time needed to measure the biomolecular interactions.