Abstract
A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 n m in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C 18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μ m as found after intrahepatic or intraperitoneal drug administration.
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