Abstract
A sensitive and rapid method for detection and quantitative determination of pyrrolizidine alkaloids is presented. It is based on stoichiometric reaction of protonated alkaloids with methyl orange, followed by a release of the latter from the complex. The color intensity of the dye is assessed visually or spectrophotometrically. The method easily detects alkaloids at a concentration of 0.5 μg/ml. The sensitivity of the quantitative assay ranges from 0.006 to 0.045 μmol/ml (from about 2 to 15μg/ml). The method proved to be especially useful during extraction, purification, and separation of minor pyrrolizidine alkaloids.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
