Abstract
The presence of luminol during lipoxygenase I-catalyzed oxygenation of linoleic acid caused a complex pattern of emission of chemiluminescent light. During the aerobic phase of the lipoxygenase reaction, the rate of formation of light was dependent on the enzyme, linoleic acid, and luminol concentrations. The correlation was linear between the rate of chemiluminescent light formation and lipoxygenase I concentration in the range 2–22 mU/ml of enzyme in the presence of saturation concentrations of linoleic acid (0.4 m m) and luminol (0.04 m m). Luminol and derivatives of isoluminol were all applicable as chemiluminescent probes without an inhibitory effect on the oxygenation rate itself. Free radicals that dissociated from the enzyme-substrate complex in the presence of oxygen in a constant ratio to the oxygenation rate are suggested to be responsible for luminol oxidation.
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