A sensitive hydrogen peroxide biosensor based on a new electron mediator 1-aminoethoxy-5-ethylphenazine dioctyl sulfosuccinate

Abstract

Hydrogen peroxide (H2O2) plays a vital role in the fields of pharmaceutical production and biomedicine. The level of H2O2 in cells directly affects the growth and apoptosis of cells, so the development of H2O2 detection technology is very important. In this paper, a new type of electron mediator, 1-aminoethoxy-5-ethylphenazine dioctyl sulfosuccinate (aePEDS) was designed and synthesized and a biosensor based on a glass carbon (GC)/aePEDS/ horseradish peroxidase (HRP) modified electrode for the detection of H2O2 was developed. H2O2 was detected by the biosensor at a low working potential of 0.11 V (vs. Ag/AgCl), avoiding interference from other electroactive substances. Under optimal conditions, the limit of detection was 0.2 μM and the sensitivity was 0.001 μA/mM. The developed sensor was used to evaluate the activity of antitumor drugs by detecting the level of hydrogen peroxide in the cell culture medium after drug stimulation of lung cancer cells. Compared with the hydrogen peroxide kit method, the constructed electrochemical biosensor can more sensitively and accurately reflect the impact of the change of hydrogen peroxide content on cells after drug stimulation and is a more promising method for cell detection and screening of drug activity.