Abstract
A sensitive fluorescence resonance energy transfer (FRET) biosensor is proposed to detect 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is a typical DNA oxidation damage product excreted in human urine. The FRET biosensor was based on carbon dots (CDs)-modified nanoporous alumina membrane with CDs as fluorescence donors. Gold nanoparticles were encapsulated in zeolitic imidazolate framework-8 to form Au@ZIF-8 nanoparticles as signal quenchers. CDs and Au@ZIF-8 nanoparticles were biofunctionalized by 8-OHdG antibody. The capture of 8-OHdG on the membrane substrates can bring Au@ZIF-8 nanoparticles closely to CDs. With 350 nm excitation, the fluorescence of CDs was quenched by Au@ZIF-8 nanoparticles and FRET effect occurred. The quenching efficiency was analyzed. The limit of detection (LOD) was 0.31 nM. Interference experiments of the FRET biosensor showed good specificity for 8-OHdG detection. The biosensor could detect urinary 8-OHdG sensitively and selectively with simple sample pretreatment processes. It shows applicability for detecting biomarkers of DNA damage in urine or other biological fluids.
Highlights
The potential toxicity of environmental pollutants and pharmaceutical candidates to human health relates to their metabolites reactive oxygen species (ROS) [1,2]
We develop a sensitive fluorescence resonance energy transfer (FRET) biosensor based on carbon dots (CDs)-modified nanoporous alumina membrane with CDs as fluorescence donors and Au@Zeolitic imidazolate framework-8 (ZIF-8) nanoparticles as signal quenchers for 8-OHdG detection in urine
FRET biosensor was constructed based on nanoporous alumina membrane with CDs and Au@ZIF-8 nanoparticles, respectively, as fluorescence donors and acceptors
Summary
The potential toxicity of environmental pollutants and pharmaceutical candidates to human health relates to their metabolites reactive oxygen species (ROS) [1,2]. Zeolitic imidazolate framework-8 (ZIF-8) is a kind of the typical nMOFs materials [18,19] It is composed of ZnN4 tetrahedral structure unit formed by zinc ion (Zn2+) and nitrogen atom (N) in methylimidazole ester. Nanoporous alumina membrane-based FRET biosensors with nanoparticles as the fluorescence donors and acceptors provide easy operation, good photostability, and sensitivity for 8-OHdG detection. CDs together with gold nanoparticles have been used to develop a FRET assay method for detecting DNA containing oxidatively damaged product 8-OHdG (DNA-8-OHdG) [38]. We develop a sensitive FRET biosensor based on CDs-modified nanoporous alumina membrane with CDs as fluorescence donors and Au@ZIF-8 nanoparticles as signal quenchers for 8-OHdG detection in urine. CDs-modified nanoporous alumina membranes were used as the biosensor substrate for 8-OHdG detection. It shows the potential applications for sensitive DNA damage biomarker detection
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