Abstract
Conventional tests for measuring the biological activity of chicken interleukin (IL)-18 require primary chicken spleen cells. We now describe a sensitive bioassay that is based on interleukin-18-induced release of interferon (IFN)-γ by a permanent chicken cell line. In B19-2D8 cells, cytoplasmically stored interferon-γ is quickly secreted in response to interleukin-18 exposure.
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