Abstract
In order to determine the blood level of 1-β- d-arabinofuranosylcytosine (Ara-C), an antileukemic agent, a sensitive and specific radioimmunoassay (RIA) system using anti-Ara-C serum, [5- 3H] Ara-C and a dextran-coated charcoal method has been developed. The anti-Ara-C serum obtained from a guinea pig was hardly cross-reactive with 1-β- d-arabinofuranosyluracil (Ara-U), tetrahydrouridine (THU) and other Ara-C analogues. The RIA system for Ara-C could detect concentrations as low as 60 pg/ml in plasma. Average of the intra- and inter-assay variancies at 5, 10, 20 ng/ml were 4.3% and 5.6%, respectively. Ara-C in blood samples obtained from human patients who orally received N 4-palmitoyl-1-β- d-arabinofuranosyl-cytosine (PL-AC) was determined by the present RIA system.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
