Abstract
A sensitive and selective immuno-nanogold resonance scattering spectral assay was developed for the determination of trace hapten penicillin G, based on the resonance scattering (RS) effect of the nanogold at 560 nm, and the nanogold-labelled immunoreaction took place in pH 5.4 phosphate citric acid buffer solutions and in the presence of polythylene glycol (PEG). The nanogold-labelled immunocomplex formed more and more with addition of penicillin G. The enhanced RS intensity at 560 nm Delta I(RS) was linear to the penicillin G concentration in the range 7.5-1700 ng/mL, with a detection limit of 0.78 ng/mL. The results indicate that the immunonanogold-labelled RS spectral assay has a high specificity and sensitivity for quantitative determination of penicillin G in raw milk samples.
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