Abstract
A highly sensitive and selective fluorescein-based probe 1 for hypochlorite anion was synthesized. The probe 1 has favorable characteristics for biological imaging, including high water solubility, high fluorescence yield, pH-independent fluorescence, and biocompatibility. Results show that it has a detection limit of 40 nM to hypochlorite anion. In addition, confocal fluorescence microscopy imaging using RAW264.7 cells showed that the new probe 1 could be used as an effective fluorescent probe for detecting HOCl in living cells.
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