A sensitive and rapid method of lead detection using nanoparticle technology based on monoclonal antibody.

Abstract

Lead (Pb) threatens public health due to its toxicity and nonbiodegradable characteristics. It is of significance to develop a sensitive and rapid method for Pb detection. In this study, monoclonal antibodies against Pb were screened with a high affinity constant (Kaff) of 3.56 × 109 L/mol. Au nanosphere particles (AuNS) and Au nanoflower particles (AuNF) were synthesized with a diameter of 15 nm and 60 nm, respectively. The specific anti-Pb antibodies were then immobilized on AuNS and AuNF for probe development. At last, AuNS- and AuNF-based strips were successfully assembled for comparative study, which were able to effectively detect environmental Pb in 10 min. The limits of detection (LODs) were determined to be 3.91 ng/ml and 0.2 ng/ml, respectively. Thus the developed method provides a feasible solution for sensitive and rapid detection of Pb on site, which is beneficial to food safety and pollution control.