Abstract
Abstract A sensitive, rapid pre-column derivatization HPLC procedure for determination of biologically important polyamines is described. Benzoylated putrescine, cadaverine, norspermidine, spermidine, norspermine and spermine were separated on reverse-phase column using 42:58 acetonitrile:water as mobile phase and detected by UV absorption at 198 nm. The detection limits were: 0.8 pmol for putrescine, 1 pmol for spermidine and 1.3 pmol for spermine. The method was successfully applied to the analysis of polyamines in small samples of unicellular organisms.
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