Abstract
Abstract A sensitive, rapid pre-column derivatization HPLC procedure for determination of biologically important polyamines is described. Benzoylated putrescine, cadaverine, norspermidine, spermidine, norspermine and spermine were separated on reverse-phase column using 42:58 acetonitrile:water as mobile phase and detected by UV absorption at 198 nm. The detection limits were: 0.8 pmol for putrescine, 1 pmol for spermidine and 1.3 pmol for spermine. The method was successfully applied to the analysis of polyamines in small samples of unicellular organisms.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
