Abstract
Although competitive protein-binding assays for corticosterone have been in use for several years, most employ cumbersome methods for the separation of bound and. free steroid. This paper describes conditions under which dextran-coated charcoal can be successfully used for this purpose. When the time of exposure to suspended charcoal was carefully controlled, approximately 90% of free steroid was adsorbed and neglible dissociation of bound steroid occurred. The assay was highly sensitive, having a standard curve ranging from 0.05 to 1.0 ng corticosterone. When known amounts of corticosterone were added to serum, 89–95% recoveries were achieved. Other steroids showed negligible cross-reactivity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
