Abstract
A new porphyrin-based sensing platform in hexadecyl trimethyl ammonium bromide (CTAB) microemulsion is developed for highly sensitive detection of theophylline. In this sensing system, the zinc-porphyrin-cinnamic acid conjugate (Zn-TPPCA) works as fluorescence probe while theophylline can decrease fluorescence intensity of the probe. Further studies indicate the linear relationship between the fluorescence quenching value and the concentration of theophylline within a given range. And the introduction of CTAB microemulsion can greatly enhance sensibility and stability of this detecting system and facilitate the detection of theophylline. On the basis above, a highly sensitive sensing platform for theophylline is created with a low limit of detection (LOD) of 0.0083 μg mL−1 under the optimal detection conditions. And further application of this method in determination of commercially available theophylline preparation shows excellent results. Subsequent studies on quenching mechanism indicate that static quenching appears between Zn-TPPCA and theophylline. Therefore, this work provides not only a highly sensitive method for determination of theophylline but also further evidence for creation of biosensors for drugs with porphyrin derivatives.
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