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Abstract
A rapid, sensitive, and reproducible method for the semiautomated determination of plasma dopa has been developed. Dopa in deproteinized plasma or tissue is isolated by cation—exchange column chromatography, and is then processed through a Technicon AutoAnalyzer and quantitated as its hydroxyindole derivative by fluorescence spectrometry. Plasma-dopa concentrations as low as 0.3 μ m can be readily determined. Plasma and erythrocyte levels in parkinsonian patients are routinely assayed by this method in our laboratory. A correlation coefficient of 0.995 was given by a comparison between this automated method and the manual method. Plasma and erythrocyte levels of dopa and 3- O-methyldopa (OMD) were measured in 14 randomly selected patients, and correlations were made between these concentrations and the dosage of levodopa.
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