A selective and sensitive fluorescence probe for Se(IV) based on fluorescence quenching of gatifloxacin

Abstract

A novel high selective and sensitive fluorescence probe termed gatifloxacin was discovered based on fluorescence “on-off” phenomenon in the presence of Se(IV). In the Tris-HCl/acetonitrile(3:7, volume ratio, Tris-HCl 0.05 mol/L, pH=7.3) system, the fluorescence intensity of gatifloxacin was linearly decreased with the concentration increase of Se(IV) in a range of 1.0×10–5―5.0×10–5 mol/L with a correlation coefficient of 0.9979(R2=0.9958) and in a range of 5.0×10–5―1.0×10–4 mol/L with a correlation coefficient of 0.9973(R2=0.9946). The detection limit of Se(IV) was 1.70×10–6 mol/L.