Abstract
Xyloglucan-specific endoglucanase inhibitor proteins (XEGIPs) are present in a wide range of dicots, where they are believed to play a role in defense from pathogens. XEGIPs are generally present as two or three copies; however, they are reported to be present as a cluster of 10 copies in potato and tomato on chromosome 1. We have identified a second set of XEGIP-encoding genes, where two inverted copies are present on chromosome 8 of potato (Solanum tuberosum L.). The same set exists in the same order on chromosome 8 of tomato (Solanum pennellii Correll). Transcript expression analysis indicates that XEGIP10 is transcribed only in roots, while XEGIP11 transcripts were not detected under normal growth conditions. Transformed potatoes (‘Bintje’ and ‘Kennebec’) overexpressing these genes did not show any changes in phenology. Foliar screening of transgenic lines for resistance to the late blight pathogen Phytophthora infestans (Mont.) de Bary did not demonstrate a large reduction in disease progression or pathogen sporulation.
Highlights
Plants produce a number of inhibitory proteins that interact with proteases, pectinases, pectin methylesterases, xylanases, and endoglucanases (Juge 2006; Pogorelko et al 2013)
During our ongoing analysis of potato xyloglucanasespecific endoglucanase inhibitor proteins (XEGIPs)-encoding genes, we identified a second set of XEGIP genes clustered on another potato chromosome
The identification of 2 new XEGIP-encoding genes in potato expands the number of XEGIPs to 12
Summary
Plants produce a number of inhibitory proteins that interact with proteases, pectinases, pectin methylesterases, xylanases, and endoglucanases (Juge 2006; Pogorelko et al 2013). Closer study indicates that inhibitory proteins can have a direct effect on the host, independent of any pathogen interactions (Alexandersson et al 2011; Jones and Perez 2014), which may help explain their up-regulation by abiotic stress. One group of inhibitory proteins is the xyloglucanasespecific endoglucanase inhibitor proteins (XEGIPs) found in dicots. These proteins were first discovered due to their interaction with a fungal endoglucanase in glycosyl hydrolase family 12 (Qin et al 2003).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
