Abstract
Nif − strains of Azotobacter vinelandii lacking active nitrogenase undergo phenotypic reversal to Nif + under molybdenum-deficient conditions. This phenotypic reversal phenomenon has been cited as evidence that A. vinelandii possesses two nitrogen fixation systems; the conventional molybdo-enzyme system and an alternative N 2 fixation system. Recent experiments with nifHDK deletion strains support this conclusion.
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