A search for mutations that affect the non-oxidative pentose phosphate pathway inSaccharomyces cerevisiae

Abstract

A number of selection strategies have been devised to obtain mutations in the non-oxidative pentose phosphate pathway in the yeastSaccharomyces cerevisiae. Some of these schemes exploit the bidirectionality of this pathway by selecting for mutants that can grow on a mixture of two carbon sources, which allow the pathway to function in opposite directions, while failing to utilize either of them alone. Other strategies select for mutations that prevent growth on glucono-5-lactone or xylulose, compounds metabolized exclusively through the pentose phosphate pathway. In the present study, these schemes yielded 35 mutants that define 11 genetic complementation groups. None of these mutations, however, affected the activity of the pathway enzymes, assayed in cell-free extracts. Nevertheless, the mutants were deficient in various aspects of carbohydrate metabolism and in the biosynthesis of the aromatic amino acids. The problems associated with the selection of mutants directly affected in the functioning of the enzymes of the non-oxidative pentose phosphate pathway have been discussed.