A scalable platform for clinical immunophenotyping: assay design and quality control for high complexity flow cytometry

Yoshinobu Koguchi,Iliana Gonzalez,Tanisha Meeuwsen,Keith S Bahjat,William Miller

doi:10.1186/2051-1426-3-s2-p251

Abstract

Meeting abstracts In the early 1980's it was demonstrated that density gradient isolation of peripheral blood mononuclear cells led to an unpredictable loss of lymphocyte populations and unacceptable levels of error for even routine CD4+ and CD8+ T cell enumeration. The error associated with this

Highlights

In the early 1980’s it was demonstrated that density gradient isolation of peripheral blood mononuclear cells led to an unpredictable loss of lymphocyte populations and unacceptable levels of error for even routine CD4+ and CD8+ T cell enumeration
Based on the recommendations of the Human Immunology Project, the assays offer results that can be correlated with other clinical sites where these recommendations are followed, while offering flexibility for characterization of additional antigens and cell populations
An unlimited number of additional antigens of interest can be quantified. This is accomplished by replicating the core cocktail as needed

Summary

Introduction

In the early 1980’s it was demonstrated that density gradient isolation of peripheral blood mononuclear cells led to an unpredictable loss of lymphocyte populations and unacceptable levels of error for even routine CD4+ and CD8+ T cell enumeration. A scalable platform for clinical immunophenotyping: assay design and quality control for high complexity flow cytometry Clinical flow cytometry laboratories have uniformly adopted whole blood lysis techniques for enumeration of leukocytes in peripheral blood. We have developed an extended menu of standardized immunophenotyping assays performed using anticoagulated whole blood.

Results

Conclusion